MICROENVIRONMENTAL SIGNALS IN HEALTH AND DISEASE
IN THE CENTRAL NERVOUS SYSTEM
Our lab’s long-standing interest focuses on how microenvironmental cues, particularly hypoxia, participate in the regulation of adaptive responses to pathological stimuli, particularly in tumor / tumor stem cell growth, cerebral ischemia and neural stem cell growth. We have dissected the differential functions of the key transcriptional regulators that mediate the hypoxia response (hypoxia inducible factor (HIF)-1alpha and HIF-2alpha) and their downstream targets in various pathological settings in the CNS
Experimentally we use a panel of glioblastoma tumor (stem) cell lines, biospies, primary human endothelial cells and murine adult neural stem cells, as well as different transgenic mouse lines, as experimental in vitro and in vivo model systems. To elucidate the role of the hypoxia response we employ a wide spectrum of methods: cloning, cell culture, biochemistry, quantitative RT-PCR, FACS, ELISA, cell-based assays (proliferation, apoptosis, migration, 3D invasion etc.), virally-based gene delivery and RNAi silencing, immunohistochemistry, in situ hybridization, light, epifluorescence and confocal microscopy, image analysis, animal models and in vivo transplantation.
We offer an open, supportive, internationally competitive academic environment with excellent training opportunities within the PhD graduate program of the International Giessen Graduate school for Life Sciences (GGL: http://www.uni-giessen.de/cms/fbz/zentren/ggl). We have won a number of external research grants and are involved in several national and international collaborative projects.
The following links provide an overview of our research projects, further details about our main areas of interest, including tumor hypoxia, tumor stem cells, and cerebral ischemia, as well as a list of selected publications.